动物营养学报2011，Vol.23Issue(2)：293-298,6.DOI:10.3969/j.issn.1006-267x.2011.02.016
枯草芽孢杆菌对Caco-2细胞抗氧化功能的影响研究
Effects of Bacillus subtilis on Antioxidative Function of Caco-2 Cells
摘要
Abstract
This experiment was conducted to study the effects of Bacillus subtilis on antioxidative function of Caco-2 cells under oxidative stress. Caco-2 cells were randomly divided into 4 groups, control Ⅰ (0 μmol/L H2O2 ) and group Ⅱ (oxidative stress group, 100 μmol/L H2O2 ) , treatment Ⅰ and treatment Ⅱ added with B.subtilis B1 and B10 (at final concentration of 0.3 × 108 CFU/mL) in the condition of oxidative stress group, respectively, whose antioxidative activities at 12 and 48 h were measured in culture supernatant and lysate. The results showed as follows: compared with the control Ⅰ and the oxidative stress group, the two groups added with B. subtilis increased significantly the total antioxidative capacity (T-AOC) in cultured supernatant of Caco-2 cells at 12 and 48 h ( P ＜ 0.01 ); B1 increased the anti-superoxide anion ( O2 - ) ( P ＜ 0.01 ), superoxide dismutase (SOD) ( P ＜ 0.01 ), catalase (CAT) activities ( P ＜ 0.01 ), and the peroxidase (POD) activity at 48 h (P ＜0.01), while B10 not only increased the activities of anti O2 -, SOD, and CAT (P ＜0.01), but also increased the inhibition capacity of hydroxyl radical ( · OH) (P ＜0.01) and the POD activity (P ＜0.05 or P ＜0.01 ); compared with the oxidative stress group, there were no significant differences in the lactate dehydrogenase (LDH) activity and malondialdehyde (MDA) content of the cells culture supernatant in the groups added with B. subtilis at 12 h (P ＞0.05), but they were significantly lower at 48 h (P ＜0.01). Compared with the control Ⅰ , there were lower SOD activity ( P ＜ 0.05), less GSH content ( P ＜ 0.05 ) and higher POD activity ( P ＜ 0.01 ) of the cells lysate in the oxidative stress group at 12 h, while the results were the contrary at 48 h,but adding B. subtilis increased POD activity significantly (P ＜0.01 ). These results indicate that the two strains of B. subtilis can increase the antioxidative function of Caco-2 cells under oxidative stress. [Chinese Journal of Animal Nutrition, 2011,23 ( 2 ): 293-298]关键词
Caco-2细胞/枯草芽孢杆菌/抗氧化活性/氧化应激/作用机制Key words
Caco-2 cell/ B. subtilis/ antioxidative activities/ oxidative stress/ mechanism分类
农业科技引用本文复制引用
崔志文,黄琴,黄怡,吴红照,文静,李卫芬..枯草芽孢杆菌对Caco-2细胞抗氧化功能的影响研究[J].动物营养学报,2011,23(2):293-298,6.基金项目
国家重点基础研究发展计划(2009CB118705),浙江省重大科技专项(2006C12086) （2009CB118705）
