寄生虫与医学昆虫学报2011，Vol.18Issue(1)：1-5,5.DOI:10.3969/j.issn.1005-0507.2011.01.001
人隐孢子虫IbA19G2基因亚型蒙古沙鼠感染模型的建立
Laboratory Model for Cryptosporidium hominis IbA19G2 Subtype in Mongolian Gerbils
摘要
Abstract
Researches have sbown that Cryptosporidium hominis is one of the main Cryptosporidium parasites for human cryptosporidiosis in China. However, only a few isolates of C. hominis were obtained and aerially sub-cultivated in laboratory up to date, so the biological characteristics of C. hominis remaina unreported in our country. In the present work, Mongolian gerbils (Meiiones unguiculataus) were orally inoculated with 0. 75 mg of dexamethasone/animal by using gastric tube drench every two days from the 1Oth day before oocyst ingestion. The results showed that the gerbils started to shed oocysts in the third day after inoculation ( DAI) , the number of shed oocysts increased obviously, reached the peak value at the 6th DAI,and then dropped graduaUy until lOth or llth DAI. The 18S rRNA and gp60 gene analysis performed before and after inoculation revealed no genetic difference of C. hominis oocysts. A stable rodent model of C. homiais Ib subtype infection has been successfully established, which may be an important value for the further study on the biological characteriatica of C. hominis.关键词
人隐孢子虫/蒙古沙鼠/免疫抑制/感染模型引用本文复制引用
王强,杨楠,菅复春,王荣军,宁长申,张龙现..人隐孢子虫IbA19G2基因亚型蒙古沙鼠感染模型的建立[J].寄生虫与医学昆虫学报,2011,18(1):1-5,5.基金项目
国家重大传染病专项重要寄生虫病检测和监测技术研究(2008ZX10004-011) （2008ZX10004-011）
河南省重大公益科研项目(81100912300) （81100912300）
