西安交通大学学报（医学版）2011，Vol.32Issue(2)：201-204,4.
Flavopiridol诱导多发性骨髓瘤细胞凋亡的分子机制
Molecular mechanisms of flavopiridol in inducing apoptosis in multiple myeloma
摘要
Abstract
Objective To study the molecular mechanisms of flavopiridol in inducing apoptosis in multiple myeloma (MM). Methods TUNEL methods were used to detect cell apoptosis; Western blot was used to determine the expression levels of myeloid cell leukemia-1 (Mcl-1), CyclinD2, Bcl-XL, Bag-1 and XIAP. Results The clinical concentration of flavopiridol resulted in rapid apoptosis after dosing within 3 h in MM cell lines.Different doses of flavopiridol could induce rapid down-expression of Mcl-1 and inhibition of CTD phosphorylation.The dose of flavopiridol's inhibition on CTD phosphorylation was closely related to the dose of its inhibition on Mcl-1expression. This dose was also the one that induced apoptosis in MM cells. Transcription inhibitor actinomycin D and flavopiridol could also inhibit the expression of Mcl-1 and induce apoptosis in primary MM. Conclusion Apoptosis of myeloma cells induced by transcription inhibitor actinomycin D and CDK inhibitor flavopiridol may be related to their inhibition on CTD phosphorylation, thereby inhibiting Mcl-1 at the mRNA and protein levels.关键词
多发性骨髓瘤/flavopiridol/Mcl-1/Bcl-XL/Bag-1/CyclinD2分类
医药卫生引用本文复制引用
王孟昌,张斌..Flavopiridol诱导多发性骨髓瘤细胞凋亡的分子机制[J].西安交通大学学报（医学版）,2011,32(2):201-204,4.基金项目
国家自然科学基金资助项目(No.81071952) （No.81071952）
