广东医学2011，Vol.32Issue(1)：50-53,4.
抑癌基因蛋白p16在宫颈癌中过度表达对其结合Cdk4功能的影响
Effects of over-expressed p16 on its binding capacity with Cdk4 in cervical squamous cell carcinoma
摘要
Abstract
Objective To observe the hinding capacity of over - expressed p16 with Cdk4 in cervical carcinoma.Methods Immunohistochemiscal staining of p16 and Cdk4 was performed on 142 samples of cervical tissues, including 19, 15 , 42 and 66 cases of cervicitis, low cervical intraepithelial neoplasm ( LCIN ) , high CIN ( HCIN ) and invasive cervical squamous cell carcinoma ( SCC ), respectively. Co - immunoprecipitation ( Co - IP ) and Western blot were used to test the activity of p16 binding Cdk4 in high p16 expression and low p16 expression SCC. Results In the lesions of cervicitis, LCIN, HCIN and SCC, the p16 positive rates were 0, 60. 0％ , 78. 6％ and 92. 4％ , respectively, with Cdk4 positive rates of 21. 1％ , 46. 7％ , 73. 2％ and 92. 4％ , respectively. The expression of p16 and Cdk4 was increasingly high with the progression of cervical tumors. In individual SCC, 83. 9％ of high p16 expression SCC had high Cdk4 expression,and 86. 7％ of low p16 expression SCC had high or parallel low Cdk4 expression, indicating that Cdk4 was adequate in most SCC. The proportion of the p16 binding with Cdk4 to the total p16 was 66. 9％ in the high p16 expression SCC while 42. 2％ for the low p16 expression SCC, indicating that half or so of the over - expressed p16 was bound with Cdk4 , and the higher p16 expressed, the more p16 was bound with Cdk4. Conclusion The over - expressed p16 in cervical carcinoma maintains the normal activity to bind Cdk4. The finding in the tissue level advances a step to further study the role of the over - expressed p16 in the carcinogenesis of cervical carcinoma.关键词
宫颈癌/p16/Cdk4/蛋白结合引用本文复制引用
庞天云,邓飞,郑晓娟,邹琳,李飞虹,胡新荣..抑癌基因蛋白p16在宫颈癌中过度表达对其结合Cdk4功能的影响[J].广东医学,2011,32(1):50-53,4.基金项目
广东省湛江科技招标项目,人事部留学人员科技项目择优资助项目,教育部留学回国人员科研起动基金(编号:教外司留[2005]383号) （编号:教外司留[2005]383号）
