现代检验医学杂志2011，Vol.26Issue(1)：45-47,3.DOI:10.3969/j.issn.1671-7414.2011.01.017
免疫透射比浊法与免疫散射比浊法检测特定蛋白的比较研究
Study on Analysing Serum Specific Proteins by Immunoturbidimetric Assays and Immunonephelometric Assays
彭凤 1应春妹 1徐晓萍 1于嘉屏1
作者信息
- 1. 上海交通大学医学院附属仁济医院检验科,上海,200127
- 折叠
摘要
Abstract
Objective To primarily evaluate the methodological performance of [mmunoturbidimetric assays for analysis of human serum specific proteins.and compare the differences with that of immunonephelometric assays. Methods Seven serum proteins (immunoglobulins G.A and M ,complement components 3 and 4,C-reactive protein, high sensitive C-reactive protein)were detected by Roche Modular P automatic biochemistry analyzer and Dade Behring BN ProSpec analyzer,and primarily methodological evaluation including precision analysis of immunoturbidimetric assays and immunonephelometric assays ,correlation and linear regression analysis of two methods was performed. Results (i)Either within-run precision or daytoday precision of immunoturbidimetric assays was very good ,coefficient of variation (CV) were both less than 5％. The precision of immunoturbidimetric assays was better than immunonephelometric assays.② Immunoturbidimetric assays and immunonephelometric assays showed good linear regression from low detection range to high detection range. The slope were all close to 1 and the intercept were all on the verge of O. The correlation coefficient of two methods was all greater than 0. 975. ③The linear assay function on the automatic biochemistry analyser could discover the problem about antigen excess of the specific samples. ④There are many advantages of immunoturbidimetric assays ,such as low cost ,low charge and high detection speed , etc. Conclusion Immunoturbidimetric assays have excellent precision , sensitivity for analysis and measuring range. It correlated well with immunonephelometric assays and had little systematic error. Moreover,it is more practical and convenient than immunonephelometric assays.关键词
免疫透射比浊法/免疫散射比浊法/方法学评价分类
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彭凤,应春妹,徐晓萍,于嘉屏..免疫透射比浊法与免疫散射比浊法检测特定蛋白的比较研究[J].现代检验医学杂志,2011,26(1):45-47,3.
