中国中医药信息杂志2011，Vol.18Issue(2)：41-43,3.DOI:10.3969/j.issn.1005-5304.2011.02.017
加味四逆散对血吸虫病小鼠肝纤维化相关因子表达的影响
Effect of Modified Sini Powder on Expression of Liver Fibrosis Related Factors in Schistosomiasis Mice
摘要
Abstract
Objectives To evaluate the effect of Modified Sini Powder on the hepatic expression of hyaluronic acid (HA), procollagen peptide (PCⅢ), hydroxyproline (Hyp) and transforming growth factor-β (TGF-β1) in a murine model of liver fibrosis induced by Schistosoma japonicum infection. Methods The NIH mice were successfully induced to liver fibrosis by infection with Schistosoma japonicum and then randomly divided into model group (group B), praziquantel group (group C), praziguanted plus Modified Sini Powder group (group D) and praziquantel plus colchicine group (group E). The mice in groups C, D and E were treated with praziquantel, praziquantel plus Modified Sini Powder and praziquantel plus colchicine, respectively. In addition, a group of unfected NIH mice (group A) served as normal control.Histological specimens were stained with HE. Expression of TGF-β1 mRNA was determined by RT-PCR and the content of Hyp in liver was measured by ELISA. Radioimmunoassay technique was employed to detect the serum content changes of HA and PCⅢ. Results The levels of HA, PCⅢ, Hyp and TGF-β1 mRNA in group D were markedly lower than those in group B, C and E (P＜O.05). Conclusion Modified Sini Powder combined with praziquantel can implove liver fibrosis due to Schistosoma japonicum, perhaps by down-regulating the expression of TGF-β1 mRNA.关键词
血吸虫病/肝纤维化/加味四逆散/透明质酸/血清Ⅲ型前胶原/羟脯氨酸/转化生长因子-β1/小鼠分类
医药卫生引用本文复制引用
李珊,蔡锐,伍参荣..加味四逆散对血吸虫病小鼠肝纤维化相关因子表达的影响[J].中国中医药信息杂志,2011,18(2):41-43,3.基金项目
湖南省科技厅资助项目(04sk1007-26) （04sk1007-26）
