中国药业2011，Vol.20Issue(2)：36-38,3.
小儿导赤片质量控制方法改进研究
Study on Determination of Rhubarb and Gardenia in Child Daochi Tablets by HPLC and Identification of Gardenia by TLC
黄云先1
作者信息
- 1. 云南省丽江市药品检验所,云南,丽江,674100
- 折叠
摘要
Abstract
Objective To establish a HPLC determination method for rhubarb and gardenia in the Child Daochi Tablets and a TLC identification method for gardenia. Methods The TLC and HPLC methods were adopted to perform the qualitative and quantitative analysis on rhubarb and gardenia in Child Daochi Tablets. Results The spots of the comparison medicinal material for gardenoside and gardenia were fairly clear by TLC identification. In HPLC quantitative analysis, the linear range of emodin was 8. 096- 104.48 μg/mL( r=0.999 9), the average recovery rate was 98.01％ ( RSD = 1.25％ ); the linear range of chrysophanol was 10. 83 - 112. 23 μg/mL( r =0. 999 9); the linear range of aloe - emodin was 8.12 - 89.90 μg/mL( r = 0. 999 8); the linear range of rhein was 9. 96 - 89.48 μg/mL( r =0. 999 8); the linear range of physcion was 8. 896 - 54.44 μg/mL ( r = 0. 999 7); the linear range of geniposide was 6. 771 - 141.48 μg/mL ( r = 1. 000 0), the average recovery rate was 99. 04％ (RSD = 1.20). Conclusion The qualitative identification method is exclusive with clear spots; the content determination method is reliable with good reproducibility, which provides the basis for the quality control and evaluation of Child Daochi Tablets.关键词
大黄素/大黄酚/芦荟大黄素/大黄酸/大黄素甲醚/高效液相色谱法/栀子/栀子苷/薄层色谱法分类
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黄云先..小儿导赤片质量控制方法改进研究[J].中国药业,2011,20(2):36-38,3.
