林产化学与工业2011，Vol.31Issue(1)：68-72,5.
绵麦冬多糖的分离纯化及自由基清除活性
Extraction, Purification and Radical Scavenging Activity of the Polysaccharides from Ophiopogon japonicus
摘要
Abstract
Crude polysaccharides (CP) were obtained from Ophiopogon japonicus ( Thumb. ) Ker-Gawl by ultrasonic extraction,followed by trichloracetic acid deproteination and ethanol precipitation. CP were further purified by DEAE-sepharose fast-flow ionexchange column chromatography. Finally the spectroscopy of CP was analyzed and the free radical scavenging activities of various fractions toward DPPH. and .OH were studied. One neutral polysaccharide(NP) and three kinds of acidic polysaccharides(AP) were separated by DEAE-sepharose FF. The result showed that NP has very low activities in scavenging DPPH. and ·OH. The ability of three kinds of AP for scavenging DPPH- and .OH were in the sequence of: AP3 ＞ AP2 ＞ AP1. AP3 has high ability of scavenging ·OH. CP has very high ability of scavenging DPPH· , and its ability of scavenging ·OH changed rapidly with the concentration change. The IC50 of various polysaccharides toward DPPH. were: AP1 22.30 g/L, AP2 9.96 g/L,AP3 7.70 g/L; CP 2.01 g/L. The IC50 of various polysaccharides toward .OH were: AP1 8.42 g/L, AP2 5.73 g/L, AP3 1.86 g/L; CP4.93 g/L.关键词
绵麦冬/多糖/分离纯化/自由基清除活性Key words
Ophiopogons japonicus ( Thumb.) Ker-Gawl/ polysaccharides/ extraction and purification/ free radical scavenging activity分类
化学化工引用本文复制引用
黄妮,熊双丽,卢飞..绵麦冬多糖的分离纯化及自由基清除活性[J].林产化学与工业,2011,31(1):68-72,5.基金项目
西南科技大学博士基金项目(06zx7122) （06zx7122）
