中国组织工程研究与临床康复2011，Vol.15Issue(6)：1079-1082,4.DOI:10.3969/j.issn.1673-8225.2011.06.028
超声联合微泡作用下即时产生非酶化一氧化氮的实验
Non-enzymatic nitric oxide induced by ultrasound mediated microbubble destruction
摘要
Abstract
BACKGROUND: Nitric oxide is one of the most important gas signaling molecule. Nitric oxide could be synthesized without nitric oxide synthetase, non-enzymatic nitric oxide, which might be one of the mechanisms of some biological effects.OBJECTIVE: To observe whether ultrasound mediated microbubbles could enhance the product by non-enzymatic pathway.METHODS: Laevo-arginine (L-Arg) was mixed with hydrogen peroxide (H2O2) by the concentration rates of 1 ∶ 1 , 10∶ 1, 10∶ 0.1,1 ∶ 10, which were irradiated with ultrasound for 60 seconds (frequency: 1 MHz, output power: 0.5, 1.0, 1 .5 W/cm2). The optimal concentration of L-Arg and H2O2 and optimal output power of ultrasound were discussed and identified. This study set up four groups, and received respectively ultrasound mediated microbubbles, simple ultrasound and microbubbles. Blank control group did not receive any intervention. Formation amount of nitric oxide was compared among groups.RESULTS AND CONCLUSION: In vitro experiment, the optimal concentration ratio of L-Arg and H2O2 was 10∶ 1, and 1.5 W/cm2 was the optimal ultrasound value. The content of nitric oxide was more in the ultrasound mediated microbubbles group than the simple ultrasound group (P ＜ 0.01). The content of nitric oxide was better in the simple ultrasound group compared with blank control group (P ＜ 0.01). No significant difference in the content of nitric oxide was detected between microbubble and blank control groups. Results suggested that ultrasound mediated microbubbles can enhance non-enzymatic synthesis of nitric oxide.关键词
超声/微泡/非酶化一氧化氮/L-精氨酸/H2O2分类
医药卫生引用本文复制引用
李鹏,童嘉毅,冯毅,杨芳,马根山,陈龙,宋佳贤..超声联合微泡作用下即时产生非酶化一氧化氮的实验[J].中国组织工程研究与临床康复,2011,15(6):1079-1082,4.基金项目
教育部2009年度高等学校博士学科点专项科研基金(20090092120 059):超声联合微泡介导内皮祖细胞心肌内归巢效应及其机制研究 （20090092120 059）
2009年度东南大学国家自然科学基金预研基金(KJ2009386):超声微泡介导内皮祖细胞心肌内归巢及其应用机制的基础研究. （KJ2009386）
