昆明医学院学报2011，Vol.32Issue(1)：30-35,6.
外源性一氧化碳对脑缺血-再灌注损伤大鼠海马气体信号分子的影响
Effect of Exogenous Carbon Monoxide on Gaseous Signaling Molecules in Hippocampus of Rats with Cerebral Ischemic-Reperfusion Injury
摘要
Abstract
Objective To investigate the effect of exogenous carbon monoxide on gaseous signaling molecules in hippocampus of rats with cerebral ischemia-reperfusion injury, and find brain protection methods. Methods 24 Wister male rats aged 3 ～ 4 months were randomly even divided into 4 groups: (Ⅰ) control group,(Ⅱ) ischemia-reperfusion group, (Ⅲ) ischemia-reperfusion+20 mL/kg CO group, ischemia - reperfusion +40 mL/kg CO (Ⅳ) group. Global cerebral ischemia-reperfusion model was established by 4-vessel occlusion.Rats in group Ⅰ was carried out sham operation. Rats in Group Ⅲ and Group Ⅳ were respectively intraperitoneally injected 20 mL/kg CO or 40 ml/kg CO before 30 min of clasping bilateral carotid artery. Rats in groupl and group Ⅱ were injected equation of saline. The carotid clasps were removed after 20 min of 4-vessle occlusion and rats were killed after 6h of reperfusion. The concentration of H2S, NO and CO, the activity of CBS, HO and iNOS,as well as the expression levels of CBS-mRNA, iNOS-mRNA and HO-l-mRNA in hippocampus of rats were measured. Results The concentration of H2S, NO and CO was increased, the activity of CBS, HO and iNOS was increased, CBS-mRNA, iNOS-mRNA and HO-l-mRNA expression was increased in group Ⅱ as compared with group Ⅰ (P＜ 0.05 or P＜ 0.01 ). The concentration of H2S and CO was increased, but NO was decreased,the activity of CBS was increased, iNOS and HO was decreased, iNOS-mRNA and HO-l-mRNA expression was decreased but CBS-mRNA was increased in group Ⅲ as compared with group Ⅱ (P ＜ 0.05 or P ＜ 0.01 ). The concentration of H2S and CO were significantly increased, but NO were significantly decreased, the activity of CBS was increased, iNOS and HO was decreased, iNOS-mRNA and HO-l-mRNA expression was decreased but CBS-mRNA was increased in group Ⅲ as compared with group Ⅱ (P ＜ 0.05 or P ＜ 0.01 ). Conclusions Exogenous CO can induce CBS-mRNA expression and inhibit HO-l-mRNA and iNOS-mRNA expression,activiate CBS and inhibit HO and iNOS during global cerebral ischemia-reperfusion in rats. CO can affect HO-l/CO, CBS/HaS and iNOS/NO systems during global cerebral ischemia-reperfusion in rats.关键词
外源性/CO/气体信号分子/缺血/再灌注/海马Key words
Exogenous/ CO/ Gaseous signaling molecule/ Ischemia-reperfusion/ Hippocampus分类
医药卫生引用本文复制引用
周银燕,万晓红,赵国良,邵建林..外源性一氧化碳对脑缺血-再灌注损伤大鼠海马气体信号分子的影响[J].昆明医学院学报,2011,32(1):30-35,6.基金项目
云南省科技厅面上基金资助项目(2008CD120) （2008CD120）
云南省教育厅科学研究基金资助项目(08C0110) （08C0110）
昆明医学院第一附属医院博士科研启动基金资助项目(2007bs10) （2007bs10）
