兰州大学学报（医学版）2011，Vol.37Issue(1)：1-5,10,6.
依托咪酯经腹外侧视前核γ-氨基丁酸能神经元产生促眠作用
Etomidate acts on γ-aminobutyric acid neurons in the ventrolateral preoptic nucleus for promoting sleep
摘要
Abstract
Objective To investigate the effect and potential targets of etomidate, a nonbarbiturate anesthetic, on sleep regulation.Methods Animals are randomly divided into the vehicle group (E1, n=6), the etomidate group before lesion (E2, n=6), vehicle group after lesion (E3,n=6), and etomidate group after lesion (E4, n=6).The methods of polysomnograph for analyzing each state time across sleep-wake cycle and Fos gene expression for determining the acting targets following etomidate administration during dark-period before and after specific cell lesion in the ventrolateral preoptic nucleus of the rat were employed in the present study.Results Etomidate administration in freely move rats induced an increase in light slow wave sleep (48.2％, P ＜0.01),deep slow wave sleep (77.7％, P ＜0.01), paradoxical sleep (83.3％, P ＜0.05) time during 12 hours (20:00-08:00), and the number of Fos-immunoreactive neurons in the ventrolateral preoptic nucleus, and a decrease in wake (35.3％, P ＜0.01) time, compared with the E1 group, respectively.However, the hypnotic effect of etomidate was attenuated after ventrolateral preoptic nucleus lesion, etomidate administration at corresponding time induced a decrease in slow wave sleep, deep slow wave sleep, paradoxical sleep time respectively by 29.1％ (P ＜0.05), 69.2％ (P ＜0.01) and 43.3％ (P ＜0.05) and the number of Fos-immunorcactive neurons in the ventrolateral preoptic nucleus, and an increase in wake time by 68.4％ (P ＜0.01) compared with the E2 group.The E2 and E3 groups after ventrolateral preoptic nucleus lesion were insignificant difference in each state across sleep-wake cycle, but they exclusively reduced deep slow wave sleep time by 44.1％(P ＜0.01) and 45.3％ (P ＜0.01) compared with the E1 group, respectively.The number of remaining Fos-immunoreactive neurons in the ventrolateral preoptic nucleus was linearly related to deep slow wave sleep time.Conclusion Etomidate acts on γ-aminobutyric acid neurons in the ventrolateral preoptic nucleus for promoting sleep.关键词
腹外侧视前核/依托咪酯/睡眠/γ-氨基丁酸/损毁Key words
ventrolateral preoptic nucleus/ etomidatc/ sleep/ γ-aminobutyric acid/ lesion分类
医药卫生引用本文复制引用
张敏,范坤,邵玉峰,赵鹏,侯一平..依托咪酯经腹外侧视前核γ-氨基丁酸能神经元产生促眠作用[J].兰州大学学报（医学版）,2011,37(1):1-5,10,6.基金项目
国家自然科学基金(30670677,81071076) （30670677,81071076）
