军事医学2011，Vol.35Issue(1)：21-24,43,5.
人源脑红蛋白转基因秀丽线虫的制备、鉴定及生理功能分析
Construction, characterization and physiological functions of transgenic C.elegans expressing human neuroglobin
摘要
Abstract
Objective To construct the transgenic Caenorhabditis elegans expressing human neuroglobin(hNgb) and to investigate the effect of hNgb on the brood size and lifespan of C. elegans. Methods The transgenic C. elegans expressing hNgb was obtained with microinjeetion. Expression of hNgb in the transgenic strains was detected by Western blot and immunohistochemistry. The life span and brood size of the C. elegans were detected using wild type N2, while the enhanced green fluorescin protein (EGFP) transgenic strain was used as a control. Results The hNgb transgenic strain was obtained. Compared with wild type C. elegans and EGFP transgenic strain, the transgenic hNgb C. elegans was no statistically significant difference in the life span and brood size. Conclusion The hNgb transgenic strain has no obvious effect on nematode's normal physiological function and could be used for the further functional identification of hNgb.关键词
脑红蛋白/秀丽隐杆线虫/转基因株系/产卵率/寿命分类
医药卫生引用本文复制引用
石锦平,任长虹,李媛,张继业,范礼斌,张成岗..人源脑红蛋白转基因秀丽线虫的制备、鉴定及生理功能分析[J].军事医学,2011,35(1):21-24,43,5.基金项目
国家973计划项目(2006CB504100),国家科技重大专项课题(2009ZX09103-616,2009ZX09301-002,2009ZX09503-002),国家自然科学基金项目(30771230,30600365),中国博士后科学基金特别资助基金(200801495) （2006CB504100）
