中国病理生理杂志2011，Vol.27Issue(3)：523-527,5.DOI:10.3969/j.issn.1000-4718.2011.03.020
肝癌SMMC-7721细胞中侧群细胞分选及其生物学特性的研究
Biological characteristics of side population cells sorted from hepatoma SMMC-7721 cell line
摘要
Abstract
AIM: To study the biological characteristics of side population ( SP ) cells sorted from hepatoma SMMC -7721 cell line.METHODS: Fluorescence - activated cell sorter ( FACS ) was used to sort SP cells and non - SP ( NSP ) cells from SMMC - 7721 cell line.The colony - formation ahility and proliferation ability between SP cells and NSP c:ells were compared in terms of plate colony assay and growth curve.The migratory and invasive properties of SP cells and NSP cells were tested by Transwell method.The cell cycle and apoptosis were analyzed by flow cytometry.The oncogenicity of the cells was analyzed hy nude mouse transplantation tumor experiment in vivo.RESULTS : The results of FACS analysis indicated that ( 9.2 ±0.2 )％ of the SMMC - 7721 cells were SP cells.The proportion of G0/G1 phase of SP cells was higher, and the apoptotic rate was lower than those of NSP cells ( P ＜ 0.05 ).The proliferation ability and colony - forming ahility and migratory and invasive properties of SP cells were significantly higher than those of NSP cells ( P ＜ 0.05 ).The nude mouse transplantation tumor experiment displayed that the oncogenicity of SP cells was higher than that of NSP cells.CONCLUSION : The SP cells sorted from SMMC - 7721 cell line may enrich tumor stem cells.关键词
肝肿瘤/侧群细胞/肿瘤干细胞/SMMC-7721细胞分类
医药卫生引用本文复制引用
黄涛,周奇,宫东伟,高全立,张旭华,吕晓东,周进学..肝癌SMMC-7721细胞中侧群细胞分选及其生物学特性的研究[J].中国病理生理杂志,2011,27(3):523-527,5.基金项目
河南省科技厅基础与前沿研究资助项目(No.082300450100) （No.082300450100）
