寄生虫与医学昆虫学报2011，Vol.18Issue(1)：15-20,6.DOI:10.3969/j.issn.1005-0507.2011.01.004
云南省新分离蚊浓核病毒的分子特征研究
Molecular Characterization of Genome of Mosquito Densovirus Newly Isolated in Yunnan Province
摘要
Abstract
To provide information for the genomic structure of mosquito densovirua ( DNV) , nucleotide of DNVs new strain isolated in Yunnan was sequenced and analyzed. Primers ( DNV3F, DNV3R) were used for genome amplification. RT-PCR was performed to amplify the fragments, which were sequenced directly and all the nucleotides were connected to acquire the genome. The computer software was used to analyze the nucleic acid data, deduce the amino acid aequence and phylogeny including ClustalX (1.8), DNAStar, GeneDoc (3.2). The 14 DNVs were isolated from Culex tritaeniorhynchus ( 10 isolates) , Anopheles sinensu ( 1 isolate ) , Cx. pseudovishnui ( 1 isolate) , Cx. annulus ( 1 isolate ) and Cx. pipiens quinquefasciatus ( 1 iaolate) of Yunnan, China. The results revealed that the new isolates were with high homology in nucleotide sequence ( 99. 9％ to 100％ ) , and in amino acid sequence (99. 7％ to 1OO％ ) , to the strains isolated from other provinces. Analysis based on the NS1 and NS2 genome sequences showed that the 14 new isolates of denaovirua from Yunnan were with the closest homology to those formerly isolated from China.关键词
浓核病毒/NS1基因组/NS2基因组/同源性分析/系统进化分析引用本文复制引用
冯云,王环宇,梁国栋,付士红,张海林,李铭华,周涛,王丕玉,邓淑珍,李金梅,王静林..云南省新分离蚊浓核病毒的分子特征研究[J].寄生虫与医学昆虫学报,2011,18(1):15-20,6.基金项目
中美虫媒病毒研究合作项目(No.U19-GH000004) （No.U19-GH000004）
国家自然科学基金资助项目(No.30560142) （No.30560142）
