重庆医学2011，Vol.40Issue(11)：1046-1048,封2,4.DOI:10.3969/j.issn.1671-8348.2011.11.003
mdr-1基因骨髓造血干细胞移植诱导大鼠肝移植免疫耐受
Induction of immune tolerance of rat liver transplantation via transplantation of mdr-1 gene bone marrow stem cells
摘要
Abstract
Objective To discuss the feasibility and possible mechanism of mdr-1 gene bone marrow stem cell transplantation inducing immune tolerance after liver transplantation.Methods Thirty male Wistar rats were used as the liver transplantation donors and 20 female SD rats as allogeneic liver transplantation recipients.The rats were randomly divided into two groups,ie, Group A (treated with mdr-l gene Hematopoietic stem cell transplantation plus liver transplantation) and Group B (sole liver transplanta tion).Ten female Wistar rats were use as syngeneic liver transplantation recipients( Group C).The survival rate, survival status,survival time and the pathological changes of the transplanted liver at one week after transplantation were compared between groups.Results The rats in Group C survived for over 60 days.The rats in Group A survived for(31.2±4.9) days, which was sig nificantly higher than (12.1±4.7) days in Group B(P＜0.05).Histologic results showed moderate to severe acute rejection,obvi ous intrahepatic inflammatory cell infiltration mainly in the portal area in Group B.Ttissue injury of the transplanted liver was significantly alleviated in Group A.There was little rejection in Group C,which was close to the normal liver tissue.Conclusion The results of the study indicate that transplantation of mdr-1 gene bone marrow stem cells can markedly alleviate the immunological rejection after rat liver transplantation.关键词
肝移植/免疫耐受/mdr-1基因引用本文复制引用
范伟,张莹,郭江富,王金龙,柳杨..mdr-1基因骨髓造血干细胞移植诱导大鼠肝移植免疫耐受[J].重庆医学,2011,40(11):1046-1048,封2,4.基金项目
贵州省省长基金资助项目[黔省专合字(2008)103] （2008）
贵州省特助基金资助项目(TZJF-2007-46) （TZJF-2007-46）
贵州省科技厅级基金资助项目(2007-2230). （2007-2230）
