南方农业学报2011，Vol.42Issue(1)：90-93,4.
促性腺激素释放激素在不同品种水牛卵巢中的免疫组化定位
Immuno—histochemical localization of gonadotropin—releasing hormone in ovaries of different buffalo varieties
摘要
Abstract
[Objective]The present experiment was conducted to investigate the similarities and differences in localization and expression of gonadotropin-releasing hormone (GnRH) in ovaries of two different buffalo varieties, viz. Guangxi swamp buffalo and Murrah hybrid buffalo, for studying the mechanism of ovary function regulation by GnRH. [Methods]The distribution and expression of GnRH and its receptors in ovaries of buffaloes were compared using immuno-histochemical SP staining. [Results]Results showed the existence of many GnRH positive cells in ovaries of both buffalo varieries, which were mainly located in cytoplasm of stratum granulosum and corpus luteum cells. The quantity of GnRH positive cells in ovaries of different buffalo varieties differed significantly, while the size and average optical density of GnRH positive cells did not show significant differences between two varieties. [Conclusion] Besides stimulating the pituitary secretions of FSH (Follicle stimulation hormome) and LH (Luteinizing hormone) in hypophysis during reproduction,GnRH might play an important role in reproductive organs to regulate the secretion of proteins, production of peptide and steroid hormone by paracrine, serf-secretion and neurosecretion, and the regulation of functional activities of these cells and organs. The difference in distribution of GnRH amongst different buffalo varieties might be related with the reproductivity.关键词
促性腺激素释放激素/卵巢/免疫组化/广西沼泽型水牛/摩拉杂交水牛Key words
GnRH/ ovary/ immuno-histochemical/ Gnangxi native swamp buffalo/ Murrah hybrid buffalo分类
农业科技引用本文复制引用
秦津,李瑞明,凌泽继,谢莹雪,宋小白,杨柄壮,许典新..促性腺激素释放激素在不同品种水牛卵巢中的免疫组化定位[J].南方农业学报,2011,42(1):90-93,4.基金项目
广西自然科学基金项目(桂科自0991042,桂科自0832043) （桂科自0991042,桂科自0832043）
广西教育基金项目(桂教科研200709LX075) （桂教科研200709LX075）
广西大学硕士研究生科研创新项目(105930903014) （105930903014）
