工业微生物2011，Vol.41Issue(1)：46-50,5.DOI:10.3969/j.issn.1001-6678.2011.01.010
红曲霉菌液相培养高产色素低产桔霉素的代谢调控研究
Metabolic regulation of liquid-phase culture of Monascus for high-yielding pigment and low-yielding citrinin
摘要
Abstract
FL1, the high Monascus pigment producing strain for liquid-phase culture was screened form 8 high Monascus pigment producing strains in solid-phase culture. The liquid-phase culture characteristics and metabolic regulation strategy for high-yielding pigment and low-yielding citrinin of FL1 were studied. Results of kinetics studies showed that the Monascus pigment and citrinin, as secondary metabolites, the relationship between their synthesis and the growth of Monascus was non-growth-coupled. The fed-batch culture technology was first used as a new method in liquid-phase culture. The biomass specific growth rate of Monascus achieved 0.21 h-1 in growth phase; in mid and late period,reducing sugar concentration could be controlled at 5 ～ 10 g/L by continuous feed. Thereby, the biomass specific growth rate of Monascus could be controled within a suitable range(0.02 h-1 ). The fed-batch technology enhanced pigment accumulation time and greatly increased the pigment production. This new culture technology had a good reproducibility and controllability. The average Monascus pigment yield rate and space time yield rate could be achieved 5 400 U/g starch and 5 625 U/(h·L), obviously more than those reported at home and abroad.关键词
红曲霉菌/红曲色素/桔霉素/分批补料Key words
Monascus/ Monascus pigment/ citrinin/ fed-batch引用本文复制引用
信亚文,郑允权,石贤爱,郭养浩..红曲霉菌液相培养高产色素低产桔霉素的代谢调控研究[J].工业微生物,2011,41(1):46-50,5.基金项目
福建省医疗器械与医药技术重点实验室开放基金项目(NO.09002.2009). （NO.09002.2009）
