南方医科大学学报2011，Vol.31Issue(1)：1-4,4.DOI:CNKI:44-1627/R.20101230.1122.000
不对称二甲基精氨酸对THP-1源性巨噬细胞表达MIF和分泌TNF-α、IL-8的影响
Effect of asymmetric dimethylarginine on MIF expression and TNF-α and IL-8 secretion in THP-1 monocytes-derived macrophages
摘要
Abstract
Objective To investigate the effect of ADMA on macrophage migration inhibitory factor (MIF) expression and tumor necrosis factor-α (TNF-α) and IL-8 secretion in THP-Ⅰ monocyte-derived macrophages. Methods THP-1 monocytes were induced to differentiate into macrophages by a 24-h incubation with 160 nmol/L PMA. The THP-1 monocyte-derived macrophages were exposed to different concentrations of ADMA for 24 h, and the changes in MIF mRNA and protein expressions were analyzed with RT-PCR and Western blotting, respectively. Enzyme-linked immunosorbent assay was used to detect the levels of TNF-α and IL-8 in the supernatant of THP-l-derived macrophages following ADMA treatments. Results ADMA obviously up-regulated MIF mRNA and protein expressions in THP-1-derived macrophages in a concentrationdependent manner. Exposure of the cells to 15 μ mol/L ADMA for 24 h showed the most potent effect in up-regulating MIF mRNA and protein expressions. ADMA treatment also resulted in a dose-dependent increase of the levels of TNF-a and IL-8 in the culture supernatant of the macrophages, and the peak levels occurred following the treatment with 15 μ mol/L ADMA.Conclusion ADMA can up-regulate MIF expression and induce TNF-α and IL-8 secretion in THP-1 monocyte-derived macrophages.关键词
不对称二甲基精氨酸/巨噬细胞/巨噬细胞移动抑制因子/肿瘤坏死因子-α/白细胞介素-8Key words
asymmetric dimethylarginine/ macrophages/ macrophage migration inhibitory factor/, tumour necrosis factor-alpha/interleukin-8分类
医药卫生引用本文复制引用
朱振东,喻卓,张旋,王庸晋,王殿华..不对称二甲基精氨酸对THP-1源性巨噬细胞表达MIF和分泌TNF-α、IL-8的影响[J].南方医科大学学报,2011,31(1):1-4,4.基金项目
Supported by National Natural Science Foundation of China (30840043).国家自然科学基金(308400043) （30840043）
