摘要
Abstract
Objective To investigate the relationship of platelet activating factor( PAF )and liver damage in rats and the therapeutic effects of ginkgo preparation. Methods 60 Wistar rats were randomly divided into four groups: normal control group( n=12 ),liver fibrosis model group( model group,n= 16 ),low dose gingko - treated group( n= 16 ), high dose gingko - treated group( n=16 ). Except the normal control group ,rats in other three groups were hypodermically injected with carbon tetrachloride( CC14 )twice a week. 12 w later, the pathological examination showed that the model of hepatic fibrosis was successfully established. After that, the nirmal control group and the model group were intragastrically administered with normal saline every day and the two treated group were perfused with Ginkgo preparation at 50 or 100 mg/( kg · d ),respectively. 12 w later,all the rats were sacrificed and their liver tissues and sera were collected. Alanine aminotransferase( ALT )and albumin( ALB ), serum PAF, hyaluronic acid( HA ), pro - collagen peptide type Ⅲ( PⅢ P ), collagen type Ⅳ( C - Ⅳ )and laminin( LN )were measured. Meanwhile, pathological examination was performed for the semiquantitative analysis of the degree of liver fibrosis. Results Compared with the normal control group, PAF, ALT, PⅢ P, C - Ⅳ, LN and HA increased significantly in the model group except that ALB reduced. Compared with the model group, all indexes in the two treated groups decreased significantly except that ALB increased, and the degree of liver inflammation and fibrosis mitigated significantly. These differences all had statistic significance. Conclusion PAF appears to be apparently associated with liver damage and liver fibrosis. Ginkgo preparation, a PAF antagonist, can obviously inhibit and reverse liver fibrosis.关键词
血小板活化因子/银杏制剂/肝纤维化/血小板活化因子拮抗剂分类
医药卫生
