中国免疫学杂志2011，Vol.27Issue(4)：300-302,307,4.DOI:10.3969/j.issn.1000-484X.2011.04.003
TLR2激动剂Pam3CK逆转IL-10转染小鼠髓样树突状细胞免疫功能的研究
TLR2 agonist Pam3CK reverses the immune function of the IL-10 transfected mouse bone marrow-derived dendritic cells
摘要
Abstract
Objective :The efFects of TLR2 agonist on the immune f'unction of IL-10 transfected bone-marrow derived dendritic cell (mDC ) .Methods :The IL-10 plasmid was transfected to mouse mDC ,and TLR2 agonist Pam3CK was added to mDC for 48 hrs .The expression of MHC Ⅱ ,CD80 ,CD86 ,FasL in mDC was detected with flow cytometry ,and the production of the IL-6 and TNF-α was detennined by ELISA . Results :IL-10 suppressed the expression of CD80 ,CD86 and MHC Ⅱ ,decreased the production of IL-6 ,TNF-α ,and increased the expression of FasL on mDC .TLR2 agonist Pam3CK increased the expression of MHC Ⅱ ,CD80 and CD86 ,enhanced the production of IL-6 and TNF-α ,but inhibited of FasL expression by the IL-10 transfected mDC .Conclusion :TLR2 activation reverses IL-10 induced low-response of mDC .关键词
IL-10/树突状细胞/TLR2激动剂分类
医药卫生引用本文复制引用
张志丽,吴砂,卢晓,弓莉,富宁..TLR2激动剂Pam3CK逆转IL-10转染小鼠髓样树突状细胞免疫功能的研究[J].中国免疫学杂志,2011,27(4):300-302,307,4.基金项目
本文为国家自然基金资助项目(C080701) （C080701）
