中国病理生理杂志2011，Vol.27Issue(2)：371-374,4.DOI:10.3969/j.issn.1000-4718.2011.02-030
不同浓度葡萄糖、胰岛素对新生大鼠心肌细胞脂联素表达的影响
Influence of glucose and insulin on expression of adiponection in neonatal rat cardiomyocytes
王梅 1杨蓉 1王亚玲 1刘金明 1李拥军 1张辉 1奇钊1
作者信息
- 1. 河北医科大学第二医院心内科,河北,石家庄,050000
- 折叠
摘要
Abstract
AIM: To study the influence of glucose or insulin at different concentrations on the expression of adiponectin in cardiomyocytes.METHODS : Myocardial cells were isolated from the hearts of neonatal Sprague - Dawley rats and primarily cultured.The cells stimulated with glucose at concentrations of 5.5, 20.25 , 30 , and 50 mmol/L respectively were divided into 5 groups and a hypertonic control group was set up.The cells treated with insulin at concentrations of 0, 10 -10 , 10 -9 , 10 -8 , 10 -7 and 10 -6 mol/L were also divided into 6 groups.The mRNA and protein levels of adiponectin( APN ) were determined by RT - PCR and Western hlotting 48 h after the intervention with glucose or insulin.RESULTS: ( 1 ) The expression of adiponectin decreased with the elevation of glucose concentration at the doses lower than 30 mmol/L( P ＜ 0.05 ).When higher than 30 mmol/L, the expression of adiponectin didn't change significantly.The osmotic pressure did not affect the adiponectin expression in cardiomyocytes.( 2 ) The expression of adiponectin decreased with the elevation of insulin concentration at the doses lower than 10 -7 mmol/L( P ＜ 0.05 ).When higher than 10-7 mmol/L, the expression of adiponectin didn't change significantly.CONCLUSION : The expression of APN decreases with the elevation of glucose or insulin concentrations within a certain range of dosage.关键词
心肌细胞/脂联素/葡萄糖/胰岛素/大鼠分类
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王梅,杨蓉,王亚玲,刘金明,李拥军,张辉,奇钊..不同浓度葡萄糖、胰岛素对新生大鼠心肌细胞脂联素表达的影响[J].中国病理生理杂志,2011,27(2):371-374,4.
