安徽农业科学2011，Vol.39Issue(3)：1315-1317,3.
小麦-黑麦易位系的细胞学和SSR检测
Cytological and SSR Detection of Wheat-Rye Translocation Line
摘要
Abstract
[ Objective] The line 07-4,the high generation offspring of crossing between 5R/5A and 6R/6A was tested. [ Method] Morphological and cytological and SSR techniques were used in the detection. [ Result ] In the field,line 07-4 grew evenly presenting favorable agronomic traits and it had big ear,multispikelets with more number of grains per ear. The chromosomes numbered 2n = 42 in its root tip-cells and the chromosome configuration was 2n =21 Ⅱ ,which formed 21 bivalents and qudravialents hybrids F PMC MI of Chinese spring wheat SSR primer pairs were used for amplification and the specific DNA fragment of rye can be amplified by primer SCM268 in line 07-4. [ Conclusion]These results preliminarily indicated that line 07-4 was a wheat-rye transloeation line.关键词
小麦/黑麦/易位系/细胞学/SSRKey words
Wheat/ Rrye/ Ttranslocation line/ Cytology/ SSR分类
农业科技引用本文复制引用
丁海燕,郑茂波,张利敏,徐英博,李集临..小麦-黑麦易位系的细胞学和SSR检测[J].安徽农业科学,2011,39(3):1315-1317,3.基金项目
国家自然科学基金项目(39670417) （39670417）
黑龙江省自然科学基金项目(C200826). （C200826）
