解剖学杂志2011，Vol.34Issue(1)：55-57,3.
人参皂甙Rg1对大鼠肢体缺血再灌注后脑组织Bax和Bcl-2表达的影响
Effect of ginsenoside Rg1 on Bax and Bcl-2 expression in the brain following limbs ischemia-reperfusion in rats
周洪霞 1张作风 1张志勇 1张连元 2张宇新1
作者信息
- 1. 华北煤炭医学院解剖学教研室,唐山063000
- 2. 华北煤炭医学院病理生理学教研室,唐山063000
- 折叠
摘要
Abstract
Objective: To evaluate Bax, Bcl-2 expression changes in brain injury after hind limbs ischemia-reperfusion (LIR) in rats, and the effect of ginsenoside Rg1 on the model of brain injury following LIR.Methods: The LIR model was established in rats.The histopathological changes were determined by Nissl staining, apoptotic cells were detected with TUNEL, the expression of associated protein, such as Bax, Bcl-2 was determined by immunohistochemistry and Western blot, and the effect of ginsenoside Rg1 was also expressed.Results: The contents of MDA following LIR increased significantly.The expression of Bax and Bcl-2 following LIR was higher than that in the control group.The expression of Bax gene was quite obvious in the red nucleus region of midbrain, 10.0 mg/kg ginsenoside Rg1 after 4 h ischemia obviously inhibited the expression of Bax.Conclusion: The excessive apoptosis and apoptosis-associated factors play a role in brain injury following LIR in rats.Ginsenoside Rgl may adjust the expressions of Bcl-2 and Bax, and thus decrease the occurrence of apoptosis.关键词
脑/再灌注损伤/细胞凋亡/Bax/Bcl-2/人参皂甙Rg1Key words
brain/ reperfusion injury/ apoptosis/ Bax/ Bcl-2/ ginsenoside Rg1引用本文复制引用
周洪霞,张作风,张志勇,张连元,张宇新..人参皂甙Rg1对大鼠肢体缺血再灌注后脑组织Bax和Bcl-2表达的影响[J].解剖学杂志,2011,34(1):55-57,3.
