中国中西医结合杂志2011，Vol.31Issue(1)：99-103,5.
益气除痰方对脾虚Lewis肺癌小鼠肿瘤生长、生存期及PRDX-1、PRDX-6表达的影响
Effects of Yiqi Chutan Recipe on Tumor Growth,Survival Time and Expressions of PRDX-1 and PRDX-6 in Lewis Lung Carcinoma Model Mice with Pi-deficiency Syndrome
摘要
Abstract
Objective To investigate the effects of Yiqi Chutan Recipe (YCR, a Chinese herbal prescription for invigorating qi and removing phlegm) on the growth and metastasis of tumor, survival time, and the expressions of peroxiredoxin ( PRDX-1 and PRDX-6) in tumor tissue of C57BL/6J mice bearing Lewis lung carcinoma.Methods Lewis lung carcinoma cells were transplanted to 90 C57BL/6J mice receiving preconditioning for inducing Pi-deficiency syndrome and divided into three groups treated respectively with saline, high dose YCR (3.0 g/kg) and low dose YCR (1.0 g/kg) once a day via gastric infusion.Besides, a group of 30 healthy mice simply received tumor cell transplantation was set up for controls.Ten mice selected from each group were sacrificed 21 days later, the size, weight and lung metastasis foci of tumor in mice were measured, and expressions of PRDX-1 and PRDX-6 in tumor tissue were detected using immunohistochemical method.The survival time of the remained 20 mice in each groups was observed.Results Tumor size,weight and the numbers of lung metastatic foci were ( 1.14 ± 0.30 ) cm3, (0.83 ± 0.26 ) g, (6.20 ± 2.53 ) foci in the high dose YCR treated group,which were significantly lower than those in the control group [(2.83 ±0.35)cm3, (2.08 ±0.28)g,and (8.60 ± 1.84) foci]respectively, also lower than those in the saline treated group [(2.29 ± 0.49 ) cm3, ( 1.67 ± 0.33 ) g and (8.70 ±2.00) foci].The median survival time in the three groups, in above order, were 29.00 ±0.89 days, 22.00 ±0.75 days and 21.00 ±0.53 days; the average survival time in them 29.60 ±0.53 days, 22.90 ± 0.50 days and 20.95 ±0.44 days; the PRDX-1 expression were 0.15 ±0.03, 0.52 ±0.07 and 0.61 ±0.09;and the PRDX-6 expression were 0.12 ±0.02,0.43 ±0.06 and 0.56 ±0.07, all showed significant difference in comparing the indices in the high dose treated group with those in the control group and in the saline treated group ( P ＜ 0.05 or P ＜ 0.01 ).The tumor growth inhibition rate was 50.30％ in the high dose YCR group with life prolongation rate of 41.29％, all better than those in the low dose YCR treated group ( P ＜ 0.05 ).Conclusions YCR can remarkably inhibit the growth and metastasis of Lewis lung carcinoma in mice with Pi-asthenia syndrome, prolong their survival period, and its mechanism is possibly related to the reduction of over expressed PRDX-1 and PRDX-6.关键词
益气除痰方/脾虚证/Lewis 肺癌/生存期/过氧化物酶-1/过氧化物酶-6引用本文复制引用
林丽珠,王淑美,周京旭..益气除痰方对脾虚Lewis肺癌小鼠肿瘤生长、生存期及PRDX-1、PRDX-6表达的影响[J].中国中西医结合杂志,2011,31(1):99-103,5.基金项目
国家自然科学基金资助项目(No.30772862) （No.30772862）
