医学分子生物学杂志2011，Vol.8Issue(1)：32-35,4.DOI:10.3870/j.issn.1672-8009.2011.01.007
半乳糖配体介导大鼠白介素10基因在大鼠肝脏内的靶向表达
Galactose Ligand-mediated Rat Interleukin-10 Gene Targeting Expression in Rats Liver
摘要
Abstract
Objective To investigate targeting expression of rat interleukin 10 ( rIL-1O ) gene in the rat liver by use of galactose ligand-mediated liposome transfection. Methods The established rIL-1O eukaryotic expression plasmid was mixed with galactose ligand-mediated liposome transfection reagent at a PEI nitrogen-to-DNA phosphate charge ratio 10 ( N/P = 10 ) . The mixture was injected via tail vein into rats. At the time points 0 h, 24 h. 7 d and 16 d after gene transfer, the expression of rIL-1O gene in the liver, kidney, spleen and lung tissue and the serum was detected by RTPCR and ELISA analysis respectively. Results After 24 h and 7 d injection. the levels of rIL1OmRNA were increased significantly in the liver. The level of rIL-1OmRN was detected in the serum at ( 107. 92 ± 12. 26 ) pg/ml after 24 h and maintained at ( 33. 2 ± 13. 15 ) pg/ml after 7 d. rILlOmRNA expression was not significantly increased in the kidney, spleen and lung tissue. Conclusion rIL-1O gene can be effectively transferred to rat liver by use of galactose ligand mediated liposome transfection. And targeting expression of rIL-1O gene in the liver can be sustained for one week.关键词
大鼠白介素10/基因转移/配体介导脂质体转染/靶向表达分类
生物科学引用本文复制引用
黄月红,陈运新,郑伟达,张莉娟,陈治新,王小众..半乳糖配体介导大鼠白介素10基因在大鼠肝脏内的靶向表达[J].医学分子生物学杂志,2011,8(1):32-35,4.基金项目
福建省自然科学基金(No.2010J05062) （No.2010J05062）
福建省卫生厅青年科研项目(No.2010-1-10) （No.2010-1-10）
