实用医学杂志2011，Vol.27Issue(12)：2111-2114,4.DOI:10.3969/j.issn.1006-5725.2011.12.005
PTEN蛋白在肺癌中表达的定量分析及临床意义
Quantitative analysis of the expression of PTEN protein in lung cancer and its clinical significance
摘要
Abstract
Objective To investigate the expression and clinical significance of PTEN protein in lung cancer. Methods The expressions of PTEN protein in lung cancer , benign pulmonary disease , and normal lung tissue were detected by immunohistorhemical SP assay , and were analyzed quantitatively by a Leica Q500MC image analysis system. The single positive intensity (positive unit , PU) was used to quantitatively analyze the expression of PTEN protein. Results The PU of the expression of PTEN protein was normally distrihuted in both normal lung tissue and benign pulmonary diseases , and was positively skew distrihuted in lung cancer. The expressions of PTEN protein in lung cancer was significantly lower than those in henign pulmonary diseases and normal lung tissue (P = 0.000). There were no significant differences among the PU values of PTEN protein in squamous carcinoma ,adenocarcinoma,large cell carcinoma and small cell carcinoma (P = 0.789). The PU values of PTEN protein in peripheral lung cancer was significantly lower than that in central-type lung cancer (P = 0.023 ) , that in lung cancer with lymph node metastases ( M = 8.68 ) was higher than that in lung cancer without lymph node metastases ( M = 5.47 ,Z = -0.894 , P = 0.058 ) . The intensity of PTEN protein expression was not associated with the patients' gender , age ,smoking habit , differentiation of the tumor and pTMN stage (P ＞ 0.05 ). There were no significant differences among the expressions of PTEN protein in lung bullae , hronchiectasis , and tuberculosis (P = 0.889). Conclusions The expression of PTEN protein in lung cancer is obviously lower than that in normal lung tissue and benign pulmonary diseases; Expression of PTEN protein is a potential valuable biomarker for differentiating lung cancer,benign pulmonary disease , and normal lung tissue.关键词
肺肿瘤/PTEN/免疫组化/定量分析Key words
Lung neoplasms / PTEN / Immunohistochemistry / Quantitative andvsis引用本文复制引用
郅程,申洪,齐文娟,周军华,李玉梅,梅娟娟,严智敏,徐小艳,林妮,陈清..PTEN蛋白在肺癌中表达的定量分析及临床意义[J].实用医学杂志,2011,27(12):2111-2114,4.基金项目
广东省科技计划项目(编号:2010B060300001) （编号:2010B060300001）
