实用医学杂志2011，Vol.27Issue(12)：2124-2127,4.DOI:10.3969/j.issn.1006-5725.2011.12.009
人子宫内膜腺上皮和基质细胞的分离与培养
Isolation and cultivation of human endometrial glandular epithelial cells and stromal cells in vitro
韩冬 1王自能1
作者信息
- 1. 510632,广州市,暨南大学附属第一医院妇产科
- 折叠
摘要
Abstract
Objective To explore a convenient method of separating and culturing human endometrial glandular epithelial cells and stromal cells in vitro. Methods Human endometrial glandular epithelial cells and stromal cells from 5 cases were separated by filtrated method and gradient centrifugation. The separate cells were identified by immunocytochemical staining and laser scanning confocal microscopy. Results The expression score of CK-19 in endometrial glandular epithelial cells was 4.16 ± 0.22 , and that of Vimentin in stromal cells was 4.24 ±0.22 in cells separated by gradient centrifugation , both were significantly different from those separated by filtrated method (P ＜ 0.05). Conclusion A simple and convenient method to separate and culture human endometrial glandular epithelial cells and stromal cells can be obtained by the modified gradient centrifugation.关键词
子宫内膜/腺上皮细胞/基质细胞/细胞培养Key words
Endometrium / Clandular epithelial cell/ Stromal cell/ Cell culture引用本文复制引用
韩冬,王自能..人子宫内膜腺上皮和基质细胞的分离与培养[J].实用医学杂志,2011,27(12):2124-2127,4.
