安徽农业科学2011，Vol.39Issue(15)：9115-9117,3.
原生质体紫外诱变选育普鲁兰酶高产菌株
Breeding of Producing Strains of High Active Pullulanase through Protoplast Mutagenesis by Ultraviolet Irradiation
摘要
Abstract
[ Objective ] The purpose was to research the protoplast production condition and mutation breeding of producing strains of acid pullulanase. [ Method ] The protoplast production and regeneration condition of Halococcus sp. Z1 was researched and its protoplast was used for breeding producing strains of high active pullulanase through ultraviolet mutagenesis. [ Result ] After the strains were cultured for 14 h, it was treated by enzymolysis with 2.8 mg/ml lysozyme at 36 C for 1.5 h, and then the formation rate and regeneration rate of their protoplasts were 87.4％ and 19.4％ resp. Nine mutants were screened out through protoplast mutagenesis by ultraviolet irridiation and the activity of their produced pullulanase was higher than that of origin strain. Among the mutants, the activity of pullulanase produced from D9-08 reached 6.37 U/ml, and compared to that of orgin strain, it was enhanced by 0.96 times. The heritage of D9-08 became stable after 10 passages. [ Conclusion ] It was feasible to breed the producing mutants of acid pullulanase through protoplast mutagenesis by ultraviolet irridiation and the breeding effect of this approach was better.关键词
普鲁兰酶/原生质体/紫外诱变Key words
Pullulanase/ Protoplast/ Ultraviolet mutagenesis分类
农业科技引用本文复制引用
周念波,李轶群,王海波,梅双喜..原生质体紫外诱变选育普鲁兰酶高产菌株[J].安徽农业科学,2011,39(15):9115-9117,3.基金项目
武汉市教育局重点资助项目(2008K019). （2008K019）
