安徽农业科学2011，Vol.39Issue(15)：8858-8860,3.
卡那霉素和潮霉素对芦竹不定芽诱导与生长的影响
The Influence of Kanamycin and Hygromacyin on Adventitious Buds Induction and Growth of Arundo donax Linn.
摘要
Abstract
[ Objective ] The sensitivity of Arundo donax Linn. to kancymycin and hygromycin under tissue culture were studied in this research, which provided basis for the construction of Arundo donax Linn. gene project construction and screening of transgenic plants. [ Method] Explants of Arundo donax Linn. were cultured for three days and then transferred to adventitious buds induction and growth medium adding with different concentration of kancymycin and hygromycin. [ Result] The result showed that both of the two kinds of antibiotics had influences on adventitious buds induction and growth. In 5 - 50 mg/L hygromycin medium, both of the induction rates and growth of adventitious bud were restrained. Hygromycin caused the death of explants in 50 - 100 mg/L concentration. For kanamycin, the concentration of 5 -50 mg/L can improve the induction rate of adventitious bud. The results showed that hygromycin was prefect for transformation of Arundo donax linn.[ Conclusion] Hygromycin restrained the induction and growth of adventitious bud under low concentration, and was suitable for the mark of transgenic choice of Arundo donax Linn.关键词
芦竹/抗生素/卡那霉素/潮霉素Key words
Arundo donax Linn./ Antibiotic/ Kanamycin/ Hygromycin分类
资源环境引用本文复制引用
彭金库,徐青青,周莉凡,韩志萍,杨志红..卡那霉素和潮霉素对芦竹不定芽诱导与生长的影响[J].安徽农业科学,2011,39(15):8858-8860,3.基金项目
浙江省大学生科技创新项目 （）
浙江省钱江人才计划项目(2009R10016). （2009R10016）
