林产化学与工业2011，Vol.31Issue(2)：48-52,5.
几种纤维素酶蛋白的双向电泳分析
Analysis of Several Kinds of Cellulases by Two-dimensional Electrophoresis
摘要
Abstract
The differences of proteins and protein content of commercial cellulases, and cellulases from Trichoderma reesei Rnt C30 produced in this laboratory were investigated by two-dimensioal electrophoresis. The results showed that the main protein of two cellulases preparation, produced in this laboratory and commercial cellulase was CBH, but they had some differences in EG.Firstly, with steam-exploded corn stover as the carbon source, the expression levels of EG Ⅰ and EG Ⅱ accounted for 18 ％ and 12 ％of the secretome in protein content respectively. With dilute acid pretreated corn stover as the earbon source, the expression levels of EG Ⅱ and EGⅡ zccounted for 7 ％ and 17 ％ of the secretome respectively. Then the expression levels of EG Ⅰ and EGⅡ in the commerciail eellulase, accounted for 6 ％ and 10 ％ of the secretome respectively. Secondly, such enzymes as XYN Ⅰ, XYN Ⅱ and XYN Ⅳ were detected in cellulase preparation from this laboratory, while commercial cellulase contained only XYN Ⅳ.The expression level of β-xylosidase( BXL)in cellulase preparations from this laboratory, was 5 times of that in commercial cellulase.关键词
纤维素酶/双向电泳/酶水解/木质纤维原料Key words
cellulase/two-dimensional elecirophoresis/ enzymatic hydrolysis /lignocellulosie materials分类
化学化工引用本文复制引用
杨静,张晓萍,勇强,余世袁..几种纤维素酶蛋白的双向电泳分析[J].林产化学与工业,2011,31(2):48-52,5.基金项目
国家自然科学基金资助项目(31070523) （31070523）
江苏省科技支撑计划项目(BE2010732) （BE2010732）
国家林业局行业公益专项资助项目(200904017) （200904017）
