中国美容医学2011，Vol.20Issue(5)：761-764,4.
骨髓间充质干细胞对自然衰老小鼠T细胞的调节作用研究
Study on effect of bone marrou mesenchymal stem cells on aging mouse T cells regulation
张磊 1石兵兵 1周碧玉 2严笠 1李艳 1曹艺美 2赵振民2
作者信息
- 1. 北京协和医学院·中国医学科学院·整形外科医院研究中心,北京,100144
- 2. 北京协和医学院·中国医学科学院·整形外科医院整形一科,北京,100144
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摘要
Abstract
Objective This is a study on effect of mouse BM-MSCs on aging mouse T cell regulation.Methods There were two groups in experiment one, aging group and young group ,the difference of CD8+ CD28+ expression between the two groups were detected with flow cytometry,according to the result of experiment one, the difference of CD8 CD28 expression of separated co-culture group, mixed co-culture group and control group,which were called experiment two,was detected with flow cytometry.Results In the aging group of experiment one,the CD8+ CD28 + expression was (4.08±0.3493)％.the CD8+ CD28+ expression of young group was (5.04±0.5741)％.There was a statistical difference.In the separated co-culture group of experiment two,we co-cultured BM-MSCs and lymphocyte in accordance with the proportion of 1∶1,and the CD8+ CD28+ expression was (10.26±1.0784)％ after 48 hours.In the mixed co-culture,we mixed BM-MSCs with lymphocyte in 6 wells plate in accordance with the proportion of 1∶1,and the CD8+ CD28+ expression was (6.42±1.5680)％ after 48 hours.In control group,the lymphocyte was clutured alone,and the CD8+ CD28+ expression was (4.08±0.3493) ％ after 48 hours.There was a statistical difference.Conclusion There were difference of CD8+ CD28+ expression between aging mouse and young mouse.BM-MSCs can take aging T cells to young state.关键词
骨髓间充质干细胞/T淋巴细胞/免疫衰老/流式细胞术Key words
bone marrow mesenchymal/ stem cell T/ lymphocytes ageing/ flow cytometry分类
生物科学引用本文复制引用
张磊,石兵兵,周碧玉,严笠,李艳,曹艺美,赵振民..骨髓间充质干细胞对自然衰老小鼠T细胞的调节作用研究[J].中国美容医学,2011,20(5):761-764,4.
