中国农业科学2011，Vol.44Issue(2)：341-348,8.DOI:10.3864/j.issn.0578-1752.2011.02.014
基于柑橘及其近缘属植物DNA条形码的叶绿体编码序列筛选
Screening Potential DNA Barcode Regions of Chloroplast Coding Genome for Citrus and Its Related Genera
摘要
Abstract
[Objective] Four coding regions of chloroplast genome of Citrus and its close relatives were analyzed in an attempt to find suitable DNA barcoding markers for species identification and lay a foundation for further study of non-coding region.[ Method ] Four chloroplast DNA regions (matK, rpoB, rpoC1 and rbcL ) of 59 Citrus accessions were sequenced, the intergeneric,interspecific, intraspecific genetic distances were calculated, and the phylogenetic tree of all the accessions tested was built based on the distance data obtained. [Result] The intergeneric and interspecific sequence variations of matK were the highest among four coding regions tested, and had significant difference from other regions studied. On the contrary, no obvious variations were found in the rpoB and rpoC1 regions. The sequence variation of rbcL was medium among the fragments sequenced. [Conclusion] The matK sequence could be used as potential candidate fragment for future DNA barcoding study of Citrus and its closely related genera.关键词
柑橘属/近缘属/DNA条形码/编码序列Key words
Citrus/ related genera/ DNA barcoding/ coding sequence引用本文复制引用
于杰,闫化学,鲁振华,周志钦..基于柑橘及其近缘属植物DNA条形码的叶绿体编码序列筛选[J].中国农业科学,2011,44(2):341-348,8.基金项目
国家自然科学基金项目(30671450)、重庆市自然科学基金项目(CSTC2009BB1119)、西南大学科研基金项目(SWU208042) （30671450）
