中国烟草学报2011，Vol.17Issue(2)：63-66,70,5.DOI:10.3969/j.issn.1004-5708.2011.02.013
片烟贮藏期霉菌的分子鉴定及杀菌剂的室内毒力测定
Identification of mold fungi in stored tobacco using molecular biology technique and toxicity of fungicides
摘要
Abstract
Five types of fungi were obtained from 108 mold tobacco samples provided by China Tobacco Hunan Industrial Corporation through isolation, purification, and preservation. The shape and size of mold fungi were observed and identified under microscope. DNA was extracted to amplifyl8S rDNA ITs by PCR and clone PCR products. Sequence was detected to identify five kinds of mold fungi including Fusarium sp., Aspergillus oryzae, Aspergillus terreus, Aspergillus niger, and Penicillium citrinum through BLAST. In laboratory bioassay, 75 ％ Dacotech, 40 ％ Dimethachlon, 10 ％ difenocoazole, and 100 ％ Dimethyl fumarate medicine were tested for the effects. Results showed that 40％ Dimethachlon was the best in inhibition spectrum and antibiotic affected at dilution of 130.32 mg/kg with inhibitory rate of over 95％.关键词
霉菌/18SrDNA/毒力测定Key words
mold fungi/ 18SrDNA/ toxicity test分类
轻工纺织引用本文复制引用
朱宏建,孙元华,刘燕娟,高必达..片烟贮藏期霉菌的分子鉴定及杀菌剂的室内毒力测定[J].中国烟草学报,2011,17(2):63-66,70,5.基金项目
国家自然科学基金项目(30900960),湖南省高校科技创新平台建设经费(09K048),湖南省烟草公司项目(10-12Aa04). （30900960）
