中国烟草学报2011，Vol.17Issue(2)：71-74,4.DOI:10.3969/j.issn.1004-5708.2011.02.015
烟草根际土壤浸出液刺激烟草疫霉产孢和接种研究
Methods for inoculation and inducing sporogenesis of Phytophthora parasitica var.nicotianae by soil suspension from tobacco rhizosphere
摘要
Abstract
The sporogenic condition of Phytophthora parasitica var. nicotianae and methods for inocultion were studied. Results showed that suspensions of peat and fertile soil can stimulate production of zoospore and zoosporagium in Phytophthora parasitica var. nicotianae. The maximum zoosporangium yields were 1.43 × 104CFU in 40 μL peat suspension and 1.06 ×104CFU, in 60μL soil suspension, respectively. The maximum zoospore yields were obtained in both 40 μL peat suspension and 40 μL soil suspension with 3.12 × 106CFU and 2.30 × 106CFU respectively. Method of inoculation was also studied and results showed that the efficiency of injection vaccination on basal stem was better than irrigating root.关键词
土壤浸出液/黑胫病/游动孢子/孢子囊/人工接种Key words
soil suspension/ tobacco blank shank/ zoospore/ zoosporagium/ inoculation分类
农业科技引用本文复制引用
杨军,田香华,罗朝鹏,奚家勤,魏春阳,金立锋,薛超群,宋纪真..烟草根际土壤浸出液刺激烟草疫霉产孢和接种研究[J].中国烟草学报,2011,17(2):71-74,4.基金项目
国家环保部资助项目"中国烟草行业温室育苗病虫草综合防治技术筛选和应用推广"(合同编号:F/III/S/10/030). （合同编号:F/III/S/10/030）
