中国畜牧兽医2011，Vol.38Issue(5)：89-92,4.
家兔MC1R基因5'-侧翼序列的克隆及测序
Cloning and Sequence of MC1R Gene 5'-Flanking Sequence in Rabbit
罗浩 1侯佳妮 1李玉梅 1闫守庆1
作者信息
- 1. 吉林大学畜牧兽医学院,吉林长春,130062
- 折叠
摘要
Abstract
The rabbit coat color as one of available genetic markers plays an important role in determining breed characteristics, purity, and crossing combination.The melanocortin-1 receptor gene (MC1R) is an important candidate gene for coat color heredity because some coat color changes in many species resulted from the nucletide sequence variants of MC1R gene.In this study,two primers (RMCR1 and RMCR2) were designed to amplify the 5’-flanking region sequences of MC1R from rabbit genome according to the known rabbit MC1R sequence(AM180881).A DNA fragment of 930 bp in length was cloned from 5'-flanking sequence of MC1R gene by an end tailed-PCR strategy, the homology of nucleotide was 99 ％ with the known rabbit MC1R sequence(AM 180881 ).关键词
家兔/毛色/MC1R基因Key words
rabbit/ coat color/ MC1R gene分类
生物科学引用本文复制引用
罗浩,侯佳妮,李玉梅,闫守庆..家兔MC1R基因5'-侧翼序列的克隆及测序[J].中国畜牧兽医,2011,38(5):89-92,4.
