中国畜牧兽医2011，Vol.38Issue(5)：93-96,4.
基于等位基因特异性PCR原理建立鸡白痢沙门氏菌PCR方法
Development PCR Assay for Detecting Salmonella pullorum Based on Allele-specific PCR
涂玉蓉 1陈建红 2任涛 2张济培 1司兴奎 2牛森2
作者信息
- 1. 佛山科学技术学院动物医学系,广东佛山,528231
- 2. 华南农业大学兽医学院,广东广州,510642
- 折叠
摘要
Abstract
Based on the nuclei polymorphism of rfbS gene sequence of Salmonella pullorum at site 237 and 598 compared with S.gallinarum,a pair of allele-specific primers were designed and synthesized,and an allele-specific PCR method for detecting S.pullorum was developed consistent with result of the serological and biochemical method and the sensitivity of the PCR assay was 18 pg DNA and 4.7× 104 CFU/mL cultural liquid.The results showed that the developed allele-specific PCR was a very rapid,sensitive and specific molecular tool for the detection and identification of S.pullorum isolates.关键词
等位基因特异PCR/鸡白痢沙门氏菌/rfbS基因Key words
allele-specific PCR / Salmonella pullorum/ rfbS gene分类
农业科技引用本文复制引用
涂玉蓉,陈建红,任涛,张济培,司兴奎,牛森..基于等位基因特异性PCR原理建立鸡白痢沙门氏菌PCR方法[J].中国畜牧兽医,2011,38(5):93-96,4.
