中国畜牧兽医2011，Vol.38Issue(5)：114-117,4.
北京油鸡Lmbr1基因第16外显子SNPs与多趾性状的相关性研究
SNPs on Exon 16 of Lmbr1 Gene and its Association with Polydactyly in Beijing-fatty Chicken
摘要
Abstract
Considering Lmbr1 is a key candidate gene of chicken polydactyly, the study on search SNPs which was influence the polydactyl. By the methods of PCR-SSCP and direct sequencing, two synonymous mutation (c. 1326A＞ G, c. 1360C＞ A) and five genotypes (AABB,aaBB,AABb, AaBb, AaBB) in exon 16 of Lmbr1 gene were detected from Beijing-fatty chicken(4- teod and 5-teod). The statistical analysis showed that the a allele gene frequencies was advantage on c. 1326A＞C locus of Lmbrl between double 5-teod and mingle-teod chicken group (0.83, 0.81),A allele gene frequencies was advantage in double 4 -teod chicken(0.53). aa genoptype was preponderance in 5-teod and mingle-teod chicken group (0. 650, 0. 625) ,Aa genotype was dominant position in double 4-teod chicken(0.42), AA was only found on 4-teod chicken group,and was not found in 5-teod and mingle-teod chicken group. B allele gene frequencies was advantage on c. 1326A＞C locus in double 5-teod, mingle-teod and double 4-toed chicken group(0.85, 0.88, 0. 76), BB genoptype was preponderance in double 5-teod, mingle-teod and double 4-toed chicken group (0. 692, 0. 750, 0. 516), and was not found bb genoptype. In the chicken group, by Hardy-Weinberg equilibrium tested, c. 1326A＞C locus was in non-equilibrium, c. 1360C＞ A locus was in equilibrium. The research showed that c. 1326A＞G was close associate with polydactyly,the site may utilize to MAS.关键词
北京油鸡/多趾/Lmbr1基因/单核苷酸多态性/相关性Key words
Beijing- fatty chicken/ polydactyly/ Lmbr 1 gene/ SNP / association分类
农业科技引用本文复制引用
杨艳,刘华贵,赵兴波..北京油鸡Lmbr1基因第16外显子SNPs与多趾性状的相关性研究[J].中国畜牧兽医,2011,38(5):114-117,4.基金项目
北京市科技合同项目"北京油鸡育种及其关键技术研究". （）
