中国畜牧兽医2011，Vol.38Issue(5)：117-120,4.
猪链球菌2型分离株精氨酸脱亚氨酸酶遗传变异分析
Analysis in Heredity and Variation of the Arginine-deiminase Gene Sequence of Streptococcus suis Serotyp 2 Isolation Strains
摘要
Abstract
The aim was to analyze the reason and epidemic trend of Streptococcus suis serotype 2, and provide theoretical basis for preventing and controlling it. According to the sequence of arginine deiminase gene published by the Genbank, The primers was designed for RT-PCR amplification of ADS isolates from Henan obtaining its DNA fragments, which were cloned into pMD18-T vector respectively and sequenced. The sequences of ADS genes were analysed by DNAStar,analysed nucleotide sequence homology and drawed phylogenetic tree. The nuclear acid analysis indicated that the homology was 94.9％ to 100％ between the isolation strains,the homology was 100％ between HN5 and HN6 from the same region,and the homology of nuclear acid sequence was 95.9％ to 99.2％ between Henan isolation strains and SX332 issued by the GenBank. The homology of ADS gene sequence was higher and genetic relationship was near during prevalence strains in the same region,and it was far in different regions.关键词
精氨酸脱亚氨酸酶/序列分析/PCRKey words
arginine-deiminase/ sequence analysis/ PCR分类
农业科技引用本文复制引用
尹国友,孙婕,陈兰英,谢朝晖,王福梅..猪链球菌2型分离株精氨酸脱亚氨酸酶遗传变异分析[J].中国畜牧兽医,2011,38(5):117-120,4.基金项目
河南城建学院基金项目(2010JYB011). （2010JYB011）
