中国兽医科学2011，Vol.41Issue(5)：441-447,7.
猪源乙型脑炎病毒HEN0701株全基因组的分子特征
Molecular characterization of complete genome of Japanese encephalitis virus HEN0701 strain isolated from swine
摘要
Abstract
Four overlapping cDNA fragments from Japanese encephalitis virus(JEV) HEN0701 strain were cloned into pCR Ⅱ-Blunt-TOPO vector, followed by determination of complete genomic cDNA sequence. The complete genome of HEN0701 strain was 10 965 nucleotides in length,including a long open reading frame from 97th to 10395th nucleotide in the genome. Comparing to 6 strains of genotype Ⅰ (G Ⅰ)JEV and 5 strains of G Ⅲ, HEN0701 had high nucleotide sequence identity with G Ⅰ strains, but low nucleotide sequence identity with G Ⅲ strains in both complete genome and each gene. However, HEN0701 had high amino acid sequence identity with both G Ⅰ and GⅢ strains in both full polyprotein and each viral protein. Phylogenetic trees based on the C,E,NS3 and NS5 gene sequences of HEN0701 and 11 JEV strains were constructed with the software MEGA 5. Four phylogenetic trees had similar topology,indicating that the four genes from JEV had the similar evolutionary trend.关键词
乙型脑炎病毒/全基因组/序列分析Key words
Japanese encephalitis virus/ complete genome/ sequence analysis分类
农业科技引用本文复制引用
郑浩,孙春清,袁世山..猪源乙型脑炎病毒HEN0701株全基因组的分子特征[J].中国兽医科学,2011,41(5):441-447,7.基金项目
上海市重点科技攻关项目(09391910800) （09391910800）
上海市自然基金项目(11ZR1446200) （11ZR1446200）
