中国兽医科学2011，Vol.41Issue(5)：453-458,6.
嗜麦芽寡养单胞菌胞外蛋白酶对斑点叉尾鮰的组织病理学影响
Histopathological effect of Stenotrophomonas maltophilia extracellular protease on Ictalurus punctatus
摘要
Abstract
A protease was purified from Stenotrophomonas maltophilia using the salt fractionation with ammonium sulfate and DEAE-Sephadex A50 gel chromatography method.The injected fish appeared different death rate after intraperitoneal injection with the purified protease.The LD50 was 3.49 μg/g.At the same time,the injected fish expressed filaments,hepata and kidneys swelling and different necrotic areas were observed.The spleens were swelling and haemorrhage.Histopathologically, hepatocytes swelled with severely vacuolar degeneration and lots of hepatocytes showed necrosis.The necrotic cells were fused and became a focal necrosis.The renal tubular epithelium expressed severe vacuolar degeneration with slightly staining and lysis.Spleen was hemorrhage and reticuloendothelial cells were necrosis.The submucosa of gastrointestinal was edema.Ultrastructural pathological study showed the amount of glycogenosome in hepata decreased a lot,chromatin of hepatocyte nucleus dissolved,mitochondria swelling and vague cristae.A lot of red blood cells infiltrated spleen and chromatin of reticuloendothelial cells disappeared.Brush border of renal tubular epithelial cells swelled, fall off,and the chromatin of nucleus reduced,mitochondria swelling and smooth endoplasmic reticulum were expanded.Scanning electron microscope found gastrointestinal mucous epithelium cells were swelling,necrosis,and fall off.The results demonstrated that the extracellular protease of S.maltophilia led the hepar, kidney, spleen, brain and gastrointestinal tract obvious pathological change.关键词
嗜麦芽寡养单胞菌/胞外蛋白酶/斑点叉尾鲴/组织病理学Key words
Stenotrophomonas maltoph ilia/ extracellular protease/ Ictalurus punctatus分类
农业科技引用本文复制引用
黄小丽,舒秀梅,汪开毓,邓永强,耿毅..嗜麦芽寡养单胞菌胞外蛋白酶对斑点叉尾鮰的组织病理学影响[J].中国兽医科学,2011,41(5):453-458,6.基金项目
教育部"长江学者和创新团队发展计划"创新团队项目(IRT0848) （IRT0848）
