中国兽医科学2011，Vol.41Issue(5)：459-463,5.
猪伪狂犬病病毒GZ-Z1株的分离鉴定
Isolation and identification of porcine pseudorabies virus GZ-Z1 strain
摘要
Abstract
To obtain a strain of pseudorabies virus(PRV),The samples of the porcine aborted fetus isolated from a hoggery in Guiyang were preprocessed, and then, were inoculated into the Vero cells. The inoculated Vero cells showed the typical cytopathogenic effect when cultured to the third generation. The virus was identified by the physicochemical experiment, transmission electron microscopy, neutralization tests, animal inoculation, and viral nucleic acid detection. The results indicated that the virus was sensitive to acid,heat,IUDR and chloroform, and could be neutralized by the PRV positive serum. By transmission electron microscopy,the mature virions with envelope in uesicae were round or ellipse with a diameter of 150 to 180 nm,and the ion in nucleus were hollow or stuffed without envelope,and the capsids were lattice arrangement in mature viral inclusion bodies. The rabbits injected with cell cultures showed the typical clinical syndrome of pruritus,palsy and death in the end. Sequencing results of PCR product indicated that the obtained sequence was gD partial sequence of PRV. All of the results indicated that the PRV strain was isolated successfully. It was named Guizhou strain.关键词
猪伪狂犬病病毒/分离/鉴定Key words
porcine pseudorabies virus (PRV)/ isolation/ identification分类
农业科技引用本文复制引用
曾智勇,甘振磊,周莉,汤德元,梁海英,刘志杰,李谦,肖超能,王彬,王凤..猪伪狂犬病病毒GZ-Z1株的分离鉴定[J].中国兽医科学,2011,41(5):459-463,5.基金项目
贵州省科学技术基金项目(黔科合J字[2007]2065) （黔科合J字[2007]2065）
贵州省农业科技攻关项目(黔科合NY字[2010]3042) （黔科合NY字[2010]3042）
