浙江农林大学学报2011，Vol.28Issue(1)：80-85,6.
山核桃脂肪代谢相关cDNA文库的构建
Construction of a fat metabolism-related cDNA library in Carya cathayensis
摘要
Abstract
Carya cathayensis is a species with a high oil content in its nuts. In addition, its oil is mostly unsaturated fatty acids. Based on differential display of genes during fruit maturity studied by DDRT-PCR, a fat metabolism-related cDNA library was constructed by SMART. Titering the unamplified library showed a titer of 5.0 × l05 pfu·mL-1, an insert size between 0.7 and 2.5 kb and 70.0％ of inserts larger than 1.0 kb; and the amplified library had a titer of 5.0 × 109 pfu·mL-1, all of the inserts larger than 500 bp, and a recombination rate of 94.5％ with 66.0％ of the inserts longer than 1 000 bp. This work has laid a foundation for studies of fat-metabolizing mechanism and regulatory network as well as discovery of relevant genes. [Ch, 7 fig. 19 ref.]关键词
经济林学/山核桃/cDNA文库/SMART/脂肪代谢Key words
cash forestry/ Carya cathayensis/ cDNA library/ SMART/ fat metabolism分类
农业科技引用本文复制引用
黄银芝,周秦,黄有军,曾燕如..山核桃脂肪代谢相关cDNA文库的构建[J].浙江农林大学学报,2011,28(1):80-85,6.基金项目
浙江省自然科学基金资助项目(Y306373) （Y306373）
浙江省森林培育重中之重学科开放基金资助项目(200609) （200609）
