中国病理生理杂志2011，Vol.27Issue(6)：1066-1070,5.DOI:10.3969/j.issn.1000-4718.2011.06.005
cPKCγ-HSP60信号通路参与小鼠脑低氧预适应
cPKCγ-HSP60 signal pathway participates in hypoxic preconditioning in mice
摘要
Abstract
AIM : To identify the proteins interacted with conventional protein kinase Cγ ( cPKCγ ) which are involved in hypoxic preconditioning ( HPC ), and to investigate the role of cPKCγ - interacted heat - shock protein 60 ( HSP60 ) in the development of HPC and ischemia ( Ⅰ ).METHODS : Healthy male BALB/c mice were randomly divided into normoxia( Norm ) and HPC groups.Based on whether middle cerebral artery occlusion ( MCAO ) was performed, the mice were divided into Norm + sham group, Norm + Ⅰ group, HPC + sham group and HPC +Ⅰ group ( all n =6 ).Immunoprecipitation, two - dimensional electrophoresis and mass spectrometry were applied to identify the proteins interacted with cPKCγ.The changes of HSP60 expression in the brain of the mice after HPC and MCAO were analyzed by Western blotting.RESULTS : The interaction of cPKCγ and HSP60 was confirmed by co - immunoprecipitation result.Compared with Norm groups, the expression level of cPKCγ - interacted HSP60 obviously increased in particulate fraction of cerehral cortex in HPC mice.In the MCAO ischemic animals, the level of HSP60 expression was significantly higher in ischemic core and penumbra in Norm + Ⅰ group and HPC +Ⅰ group than that in Norm + sham group.HSP60 expression in ischemic core was lower in HPC +Ⅰ group than that in Norm + Ⅰ group.CONCLUSION: cPKCγ - HSP60 signal pathway might be involved in the development of cerebral hypoxic preconditioning in MCAO mice.关键词
常规型蛋白激酶Cγ/蛋白质组/热休克蛋白60/低氧预适应/大脑中动脉阻塞Key words
Conventional protein kinase Cγ/ Proteome/ Heat - shock proteins 60 / Hypoxic preconditioning/Middle cerebral artery occlusion分类
医药卫生引用本文复制引用
蒋淑君,张楠,李俊发..cPKCγ-HSP60信号通路参与小鼠脑低氧预适应[J].中国病理生理杂志,2011,27(6):1066-1070,5.基金项目
山东省卫生发展科技计划资助项目(No.2009HW006) （No.2009HW006）
