中国病理生理杂志2011，Vol.27Issue(6)：1084-1089,6.DOI:10.3969/j.issn.1000-4718.2011.06.008
Akt抑制剂perifosine抑制胃癌细胞增殖并诱导凋亡的实验研究
Perifosine inhibits cell proliferation and triggers apoptosis in human gastric cancer cell line SGC-7901 cells
摘要
Abstract
AIM: To investigate the effect of perifosine, a novel inhibitor of Akt, on the cell proliferation and apoptosis in human gastric cancer cell line SGC -7901.METHODS: Cell growth inhibition was detected by MTT assay.Cell cycle was analyzed by flow cytometry.Annexin V - FITC apoptosis detection kit was used to determine apoptosis in the cells.Protein expression was examined by Western blotting.RESULTS: Akt phosphorylation was dose - dependently inhibited by perifosine in SGC - 7901 cells.The results of MTT and cell cycle analysis indicated that perifosine inhibited the growth of human gastric cancer cells in a dose - dependent manner.Perifosine arrested the cell cycle progression at G2 phase.Apoptosis induction became more effective with increasing the concentration of perifosine.The caspase cascade and its downstream effector poly( ADP - ribose ) polymerase ( PARP ) were also activated upon perifosine treatment, and the level of Bcl -2 was down - regulated, whereas the protein level of Bax was up - regulated.CONCLUSION: The small molecule Akt inhibitor perifosine shows substantial anti - tumor activity in human gastric cancer cell line SGC -7901.Perifosine induces caspase - dependent apoptosis, and the key regulators include caspase - 3 , caspase - 9 and Bcl - 2.关键词
胃肿瘤/Perifosine/PI3K/Akt通路/细胞凋亡/半胱氨酸天冬氨酸蛋白酶类/Bcl-2Key words
Stomach neoplasms/ Perifosine/ PDK/Akt pathway/ Apoptosis/ Caspases/ Bcl -2分类
医药卫生引用本文复制引用
费洪荣,陈洪蕾,辛晓明,赵雪梅,王凤泽..Akt抑制剂perifosine抑制胃癌细胞增殖并诱导凋亡的实验研究[J].中国病理生理杂志,2011,27(6):1084-1089,6.基金项目
国家自然科学基金资助项目(No.30800422) （No.30800422）
泰山医学院自然科学基金资助项目(No.2010ZR081) （No.2010ZR081）
