中国病理生理杂志2011，Vol.27Issue(6)：1109-1114,6.DOI:10.3969/j.issn.1000-4718.2011.06.012
阿霉素预处理的小鼠红白血病细胞降低红白血病细胞的成瘤性
Decreasing tumorigensis of mouse erthroleukemic cells by pre-infusion of adriamycin-treated erthroleukemic cells in mice
杨理婷 1吴秉毅 1苏嘉霖 2郭坤元1
作者信息
- 1. 南方医科大学珠江医院,血液科,广东,广州,510282
- 2. 南方医科大学珠江医院,内分泌科,广东,广州,510282
- 折叠
摘要
Abstract
AIM: To investigate the effect of decreasing tumorigensis of mouse erthroleukemic ( MEL ) cells by pre - infusion of adriamycin( ADM ) - treated MEL cells in mice.METHODS: The immungentic cell death of MEL cells was prepared by pre - treatment with ADM in vivo.The Kunming ( KM ) mice were divided into 3 group: MEL model group, ADM treatment group and control group.The mice in MEL model group were intravenously injected with PBS on the first day, and then with 1 x 10 MEL cells on the 8th day.The mice in ADM treatment group were intravenously injected with 9 × 106 MEL cells pretreated with ADM on the first day, and then with 1 × 106 MEL cells without ADM pretreatment on the 8th day.The mice in control group didn't receive any intervention on the first day and 8th day.The survival time, the white cell count in peripheral blood and body weight were observed.RESULTS: All mice in control group were survived.All mice in MEL model group died from erythroleukemia.Only 1 mouse in ADM treatment group survived on the 80th day.The survival time in control group, MEL model group and ADM treatment group was 80 d, ( 28.00 ± 8.43 ) d and ( 56.00 ± 12.68 ) d, respectively.The survival time in ADM treatment group was longer than that in MEL model group ( P ＜ 0.01 ).The peripheral blood white cell count in ADM treatment group was lower than that in ADM model group ( P ＜0.01 ).The body weight of the mice in ADM treatment group on the 80th day was higher than that in MEL model group ( P ＜ 0.01 ).CONCLUSION: The pre - infusion of adriamycin - treated MEL cells in erythroleukemic mice attenuates the tumorigenesis of MEL cells and improves survival time and life quality.关键词
阿霉素/小鼠红白血病细胞/细胞凋亡/免疫原性死亡Key words
Adriamycin/ Mouse erythroleukemic cells/ Apoptosis/ Immunogentic cell death分类
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杨理婷,吴秉毅,苏嘉霖,郭坤元..阿霉素预处理的小鼠红白血病细胞降低红白血病细胞的成瘤性[J].中国病理生理杂志,2011,27(6):1109-1114,6.
