中国兽医科学2011，Vol.41Issue(5)：526-529,4.
鹅γ-干扰素基因真核表达载体的构建及在幼仓鼠肾细胞中的表达
Construction of goose gamma-interferon gene eukaryotic expression vector and its expression in BHK-21 cell lines
摘要
Abstract
The IFN-γ gene was inserted into the eukaryotic expression vector pcDNA3. 1( + ),and the eukaryotic expression plasmid pcDNA3. 1 (+)-IFN-γ was constructed and identified by sequencing. Electroporation instrument was set to 425 V of voltage and 25μF of capacitor,then pulsed 2 times consecutively under room temperature. 20μg/mL plasmid pcDNA3.1 (+)- IFN-γ was transformed into 1 × 106cells/mL.BHK-21 in 400 μL of volume. After consecutive selection by 600 μg/mL G418 for 14 days, resistant cell clones were selected,and the cell supernatants were identified by Western-blot test. The results indicated that goose IFN-γ was expressed in BHK 21 cells.关键词
鹅/γ-干扰素基因/幼仓鼠肾细胞/电穿孔/真核表达Key words
goose/interferon-γ gene/BHK-21 cell lines/electroporation/eukaryotic expression分类
农业科技引用本文复制引用
胡晓静,刘棋,付薇,徐贤坤,熊毅..鹅γ-干扰素基因真核表达载体的构建及在幼仓鼠肾细胞中的表达[J].中国兽医科学,2011,41(5):526-529,4.基金项目
广西科技攻关项目(桂科攻0719004-3G) （桂科攻0719004-3G）
