中国兽医科学2011，Vol.41Issue(5)：530-535,6.
供体细胞形态、直径和传代次数对猪体细胞核移植效果的影响
Influence of morphology, diameter and passage of the donor cells on porcine somatic cell nuclear transfer
摘要
Abstract
Oocytes on the surface of porcine ovaries were collected, in vitro matured and enucleated.Donor cells were dissected, cultured and passaged.The donor nucleus was transfered into the enucleated oocyte to reconstruct an embryo.The reconstructed embryos were fused and activated in order to discuss the developmental potential of the reconstructed embryos in the morphology and diameter and passage of donor cells and explore the transfer effect in the porcine somatic cells nuclear transfer.In result ,the cleavage rate and blastocyst rate of reconstructed embryos using smooth granulosa cells as donor cells were higher than those of the rough granulosa cells as donor cells,and it were significantly different(P＜0.05).Fusion rates in two groups were no significantly different(P＞0.05).The fusion rate of reconstructed embryos using diameter ＜15μm donor cells was lower, and it was significantly different compared with the fusion rate of the diameter ＞30μm group(P＜0.05).The cleavage rate of the diameter of 20 to 30 μm group was significant compared with the one of the diameter ＜15 μm group (P＜0.05) ,but not significant compared with the diameter ＞30 μm group(P＞0.05).The cleavage rate and blastocyst rate(63.73％ & 21.54％)of reconstructed embryos using diameter of 20 to 30 μm as donor cells were higher than other groups,and the blastocyst rate was significantly different(P＜0.05).The efficiency of nuclear transfer in different culture passages of fetal fibroblast after serum starvation was examined.The fusion rate of 6 to 9 culture passages was higher than those of 3 to 5 culture passages and over 10 culture passages, but the cleavage rate and blastocyst rate among the three groups were not significantly different(P＞0.05).In conclusion,the smooth granulose cells as donor cells were beneficial for the development of the constructed embryos,donor cells of the diameter from 20 to 30 μm were better fit for porcine somatic cell nuclear transfer,and fetal fibroblast from 6 to 9 culture passages were better suitable for as donor cells of nuclear transfer.关键词
猪卵母细胞/体细胞核移植/供体/重构胚/效果Key words
porcine oocyte/ somatic cell nuclear transfer/ donor/ reconstructed embryos/ effect分类
农业科技引用本文复制引用
黄雅琼,石德顺,陈旭健,李家洲,曾诗媛,詹萍,阮桂文,赵仕花..供体细胞形态、直径和传代次数对猪体细胞核移植效果的影响[J].中国兽医科学,2011,41(5):530-535,6.基金项目
广西自然科学基金项目(2010GXNSFA013105) （2010GXNSFA013105）
广西壮族自治区教育厅科研项目(200911MS203) （200911MS203）
玉林师范学院重点项目(2009YJZD01) （2009YJZD01）
国家高技术研究发展计划(863)项目(2007AA100505,2008AA101006) （863）
