首页|期刊导航|中华医学杂志（英文版）|Optimization of transfection efficiency of small interfering RNA in purified human prolactinoma cells
中华医学杂志（英文版）2011，Vol.124Issue(12)：1862-1869,8.DOI:10.3760/cma.j.issn.0366-6999.2011.12.018
Optimization of transfection efficiency of small interfering RNA in purified human prolactinoma cells
Optimization of transfection efficiency of small interfering RNA in purified human prolactinoma cells
摘要
Abstract
Background Control of hypersecretion of certain hormones is one of the key targets in the treatment of pituitary adenomas. RNA interference has been shown to inhibit protein expression, and thus it may represent a promising method for the treatment of pituitary adenomas. In the present study, transfection efficiency of small interfering RNA (siRNA) was optimized in human prolactinoma cells.Methods First, a method was optimized to extract highly purified human prolactinoma cells in vitro. The extracted cells were verified to retain the physiological features of prolactin (PRL) secretion. Second, three conditions for siRNA transfection were tested by the evaluation of transfection efficiency and cell viability. The proper transfection condition was verified for human prolactinoma cells. Third, the siRNA for prolactin was transfected into the human prolactinoma cells, and the suppression of PRL mRNA was evaluated by quantitative real-time reverse transcription-PCR.Conclusion It is possible to inhibit hormone hypersecretion by RNA interference, that may eventually enable therapeutic siRNA drugs developed.关键词
RNA interference/prolactinoma/prolactin/cell culture techniques/transfectionKey words
RNA interference/prolactinoma/prolactin/cell culture techniques/transfection引用本文复制引用
MENG Qing-hu,SONG Yong-mei,ZHAO Jiang,YU Chun-jiang,ZHAN Qi-min..Optimization of transfection efficiency of small interfering RNA in purified human prolactinoma cells[J].中华医学杂志（英文版）,2011,124(12):1862-1869,8.基金项目
This work was supported by grants from "973" National Key Fundamental Research Program of China (No.2002 CB513101 and No.2006 CB910602). （No.2002 CB513101 and No.2006 CB910602）
