安徽农业大学学报2011，Vol.38Issue(3)：333-336,4.DOI:CNKI:34-1162/S.20110419.0925.020
铜对牛卵母细胞体外成熟及体外受精胚胎发育的影响
Effects of copper on bovine oocyte maturation and embryo development by in vitro fertilization
摘要
Abstract
This study was to investigate the effects of chloratum culture-medium on bovine oocytes maturation and embryo development by in vitro fertilization. The concentrations of copper were mensurated by plasma atomic spectrometric techniques. Copper concentrations in culture medium were 0 mg/l (control), 0.46 mg/l and 0.68 mg.L-１. The results were as follows: there were no significant differences in oocytes maturation and 2-cell zygotes between copper and control, and copper increased the rate of morulae and blastocysts (P＞0.05). At 0-22 h of oocytes maturation, 0-48 h, 48-96 h, 96-144 h and 144-192 h of zygotes culture, decreased percentage of copper concentrations in 0.46 mg/1 treatment were 4.3％, 8.7％, 26.0％, 28.3％ and 30.4％, respectively. Decreased percentage of copper concentrations in 0.68 mg.L-1 treatment were 2.9％, 7.4％, 23.5％, 25.0％ and 27.9％, respectively.In conclusion, copper played an important role in the success of culture of morulae and blastocysts, and there had adscendent demand for copper utilized by zygotes after 48 h of in vitro zygotes culture.关键词
牛/卵母细胞/胚胎/铜/体外受精Key words
bovine/oocytes/embryos/copper, in vitro fertilization分类
农业科技引用本文复制引用
高国龙,易建明,张敏,杨利国..铜对牛卵母细胞体外成熟及体外受精胚胎发育的影响[J].安徽农业大学学报,2011,38(3):333-336,4.基金项目
国家自然科学基金(30771549/C02030104)和贵州省科学技术基金([2008]2263号)共同资助. （30771549/C02030104）
