军事医学2011，Vol.35Issue(6)：454-458,5.
利用气质联用方法研究功能性消化不良患者血浆代谢谱的变化
Metabolic changes in plasma of patients with functional dyspepsia using GC-MS
摘要
Abstract
Objective To investigate the characteristics of plasma metabolites in functional dyspepsia ( FD ). Methods Gas chromatography/time-of-flight mass spectrometry ( GC/TOF-MS )-based metabonomics was applied to compare the plasma metabolic difference between FD female patients ( n = 16 ) and normal female subjects ( n = 14 ). The integral data were subject to partial least squares-discriminant analysis ( PLS-DA ) through SIMCA-P1O. 0 software. Results Using GC/TOFMS technique , a total of 122 chromatographic peaks were detected,35 of which were identified as endogenous metabolites.Multivariate statistical analysis showed significant changes in FD plasma metabolites, such as those of urea, glycine , phenylalanine, valine, linoleic acid, cholesterol compared to healthy controls. Analysis of metabolic pathway found that FD female patients had a higher level of energy metabolism than controls. Besides, metabolism of amino acids, fatty acids and intestinal flora were affected in FD female patients. Conclusion The metabonomic approach can evaluate the level of plasma metabolites in FD patients. This new method may he of great benefit to the pathogenesis research of FD.关键词
功能性消化不良/代谢组学/气相色谱-飞行时间质谱/多元统计分析Key words
functional dyspepsia/ metabonomics/ GC/TOF-MS/ multivariate analysis分类
医药卫生引用本文复制引用
刘娟,吴巧凤,孙博,周思远,余曙光,董方霆,颜贤忠..利用气质联用方法研究功能性消化不良患者血浆代谢谱的变化[J].军事医学,2011,35(6):454-458,5.基金项目
国家973计划项目(2006CB504501) （2006CB504501）
